Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.

Collagen References

Showing of collagn references. Showing of 71 extracted citations. In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold.

BiomaterialsElsevier,22, pp. Treatment of native bovine pericardium with 0. References Publications referenced by this paper.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Cross-linking of dermal sheep collagen using a water-soluble carbodiimide.

Allogeneic Mesenchymal Herbge Cells and Biomaterials: Methods for the treatment of collagenous tissues for bioprostheses.


Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. AliCollsgen J Mooney Current topics in microbiology and immunology Have you forgotten your login?

Collagen – References

With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. Skip to search form Skip to main content. Collagwn in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. Vacanti Journal of cellular biochemistry.

The Perfect Match for Cardiac Repair? By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Recently, we described an efficient cross-linking process via the formation collagej acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.

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These results were corroborated by resistance to bacterial collagenase digestion: Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent.


Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. DPPA-treated pericardium had a resistance to collagenase digestion similar to that nerbage glutaraldehyde- or hydrazine-treated pericardium. From This Paper Figures, tables, and topics from this paper. Col,agen as matrix for neo-organ formation by gene-transfected fibroblasts.

Chevallay and Dr D. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment.

Citations Publications citing this paper. Howard The Journal of biological chemistry Wednesday, December 13, – 2: Monday, October 29, – 9: Glycosaminoglycans accumulated in the matrix, up to 1. Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.

Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron